Search results for "bacterial virulence"

showing 6 items of 6 documents

Bacteriophage Adherence to Mucus Mediates Preventive Protection against Pathogenic Bacteria

2019

The mucosal surfaces of animals are habitat for microbes, including viruses. Bacteriophages—viruses that infect bacteria—were shown to be able to bind to mucus. This may result in a symbiotic relationship in which phages find bacterial hosts to infect, protecting the mucus-producing animal from bacterial infections in the process. Here, we studied phage binding on mucus and the effect of mucin on phage-bacterium interactions. The significance of our research is in showing that phage adhesion to mucus results in preventive protection against bacterial infections, which will serve as basis for the development of prophylactic phage therapy approaches. Besides, we also reveal that exposure to m…

medicine.medical_treatmentvirusesbacteriophage therapymedicine.disease_causebakteeritBacteriophageFish Diseaseshost-pathogen interactionslimakalvotPathogenOrganism1183 Plant biology microbiology virology11832 Microbiology and virology2. Zero hunger0303 health scienceshostpathogen interactionsbiologyvirulenssimucosal pathogensQR1-5023. Good healthBACTERIOPHAGEResearch ArticleProtein BindingbacteriophagesPhage therapyeducationvirusFlavobacteriumMicrobiologybakteriofagitHost-Microbe BiologyMicrobiologyViral Proteins03 medical and health sciencesImmunityVirologyAntibiosismedicineAnimalsPhage Therapy030304 developmental biologyMucous MembraneBacteria030306 microbiologybacterial virulenceMucinPathogenic bacteriaEditor's Pickkalatauditbiology.organism_classificationMucusfagiterapiaMucusFlavobacterium columnareBacteriamBio
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Targeting Bacterial Sortase A with Covalent Inhibitors: 27 New Starting Points for Structure-Based Hit-to-Lead Optimization.

2019

Because of its essential role as a bacterial virulence factor, enzyme sortase A (SrtA) has become an attractive target for the development of new antivirulence drugs against Gram-positive infections. Here we describe 27 compounds identified as covalent inhibitors of

0301 basic medicineStaphylococcus aureusMagnetic Resonance SpectroscopyAntivirulenceVirulence Factors030106 microbiologySmall Molecule Libraries03 medical and health sciencesMiceBacterial ProteinsCatalytic DomainDrug DiscoveryAnimalschemistry.chemical_classificationBinding SitesChemistryHit to leadFibroblastsAminoacyltransferasesAnti-Bacterial AgentsMolecular Docking SimulationCysteine Endopeptidases030104 developmental biologyInfectious DiseasesEnzymeBiochemistryCovalent bondSortase ABacterial virulenceNIH 3T3 CellsStructure basedACS infectious diseases
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Helicobacter pylori gamma-glutamyl transpeptidase and vacuolating cytotoxin promote gastric persistence and immune tolerance

2013

Infection with the gastric bacterial pathogen Helicobacter pylori is typically contracted in early childhood and often persists for decades. The immunomodulatory properties of H. pylori that allow it to colonize humans persistently are believed to also account for H. pylori ’s protective effects against allergic and chronic inflammatory diseases. H. pylori infection efficiently reprograms dendritic cells (DCs) toward a tolerogenic phenotype and induces regulatory T cells (Tregs) with highly suppressive activity in models of allergen-induced asthma. We show here that two H. pylori virulence determinants, the γ-glutamyl transpeptidase GGT and the vacuolating cytotoxin VacA, contribute critic…

TransgeneVirulenceMice Transgenicbacterial virulence factorspersistence strategieshygiene hypothesisMicrobiologyImmune toleranceMice03 medical and health sciences0302 clinical medicineBacterial ProteinsIn vivoImmune ToleranceAnimalsGamma-glutamyltransferasehuman microbiotaPathogen030304 developmental biology0303 health sciences1000 MultidisciplinaryMultidisciplinaryHelicobacter pyloribiologyStomach10061 Institute of Molecular Cancer Researchgamma-GlutamyltransferaseBiological SciencesHelicobacter pyloribiology.organism_classificationbacterial infections and mycosesCoculture TechniquesIn vitrodigestive system diseases3. Good healthMice Inbred C57BLImmunologybiology.protein570 Life sciences; biology030211 gastroenterology & hepatologypersistent bacterial infection
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Diversity, virulence, and antimicrobial resistance of the KPC-producing Klebsiella pneumoniae ST307 clone

2017

ABSTRACT : The global spread of Klebsiella pneumoniae producing Klebsiella pneumoniae carbapenemase (KPC) has been mainly associated with the dissemination of high-risk clones. In the last decade, hospital outbreaks involving KPC-producing K. pneumoniae have been predominantly attributed to isolates belonging to clonal group (CG) 258. However, results of recent epidemiological analysis indicate that KPC-producing sequence type (ST) 307, is emerging in different parts of the world and is a candidate to become a prevalent high-risk clone in the near future. Here we show that the ST307 genome encodes genetic features that may provide an advantage in adaptation to the hospital environment and t…

0301 basic medicineSettore MED/07 - Microbiologia E Microbiologia Clinicasiderophoreantibiotic resistancelong term survivalsequence analysisKlebsiella pneumoniaepolymerase chain reactionResponses to Human InterventionsDrug ResistanceGene TransferClone (cell biology)ST259bacterial proteinvirulence factorYersiniabactinGenomechemistry.chemical_compoundMicrobialPlasmidAntibioticsbacterial genomepathogenicitygenetics610 Medicine & healthgenome analysisCross InfectionMolecular EpidemiologyGenomeVirulencebiologydrug effectyersiniabactinBacterialDrug Resistance MicrobialGeneral MedicineKlebsiella infectionglycogen synthesisKlebsiella pneumoniaeEnglandItalyST307horizontal gene transferProteínas BacterianasResearch ArticleGene Transfer HorizontalVirulence FactorsSequence analysiscapsule030106 microbiologyVirulence610 Medicine & healthpulsed field gel electrophoresisColombiaCarbapenemase; siderophore; yersiniabactin; bacterial protein; beta lactamase; virulence factor antibiotic resistance; Article; bacterial strain; bacterial virulence; bacterium isolate; fimbria; genome analysis; glycogen synthesis; Klebsiella pneumoniae; long term survival; microbial diversity; nonhuman; plasmid; polymerase chain reaction; pulsed field gel electrophoresis; sequence analysis; whole genome sequencing; antibiotic resistance; bacterial genome; carbapenem-resistant Enterobacteriaceae; Colombia; cross infection; drug effect; England; genetic variation; genetics; horizontal gene transfer; human; Italy; Klebsiella infection; microbiology; molecular epidemiology; multilocus sequence typing; pathogenicity; virulence Bacterial Proteins; beta-Lactamases; Carbapenem-Resistant Enterobacteriaceae; Colombia; Cross Infection; Drug Resistance Microbial; England; Gene Transfer Horizontal; Genetic Variation; Genome Bacterial; Humans; Italy; Klebsiella Infections; Klebsiella pneumoniae; Molecular Epidemiology; Multilocus Sequence Typing; Virulence; Virulence Factors; Whole Genome SequencingArticlebeta-Lactamasesbeta lactamaseHorizontalMicrobiologyCarbapenemase03 medical and health sciencesAntibiotic resistanceBacterial ProteinsplasmidHumanshumanInfecciones por KlebsiellafimbrianonhumanWhole Genome Sequencingbacterial virulencebacterium isolatemicrobiologyGenetic Variationbacterial strainbiology.organism_classificationKlebsiella InfectionsEnterobacteriaceae Resistentes a los CarbapenémicosKPCCarbapenem-Resistant Enterobacteriaceae030104 developmental biologychemistrymicrobial diversityEpidemiología MolecularGenome BacterialWGSMultilocus Sequence Typing
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Coincidental loss of bacterial virulence in multi-enemy microbial communities.

2014

The coincidental virulence evolution hypothesis suggests that outside-host selection, such as predation, parasitism and resource competition can indirectly affect the virulence of environmentally-growing bacterial pathogens. While there are some examples of coincidental environmental selection for virulence, it is also possible that the resource acquisition and enemy defence is selecting against it. To test these ideas we conducted an evolutionary experiment by exposing the opportunistic pathogen bacterium Serratia marcescens to the particle-feeding ciliate Tetrahymena thermophila, the surfacefeeding amoeba Acanthamoeba castellanii, and the lytic bacteriophage Semad11, in all possible combi…

Ecological selectionBacteriophageNatural SelectionBacteriophagesANTAGONISTIC COEVOLUTIONLISTERIA-MONOCYTOGENESSerratia marcescens1183 Plant biology microbiology virologyGeneticsSERRATIA-MARCESCENSAcanthamoeba castellanii0303 health sciencesMultidisciplinaryEcologybiologyQTetrahymenaRAcanthamoeba castellaniiMedicineResearch ArticleEvolutionary ProcessesVirulence FactorsAntagonistic CoevolutionScienceMicrobial ConsortiaeducationVirulenceMicrobiologyMicrobial EcologyMicrobiologyEvolution Molecular03 medical and health sciencesmulti-enemy microbial communitiesWater environment030304 developmental biologySTAPHYLOCOCCUS-AUREUSEvolutionary BiologyPSEUDOMONAS-AERUGINOSA VIRULENCE030306 microbiologybacterial virulenceDICTYOSTELIUM-DISCOIDEUMBiology and Life SciencesBacteriologybiology.organism_classificationOrganismal EvolutionArtificial SelectionTETRAHYMENA-THERMOPHILAEvolutionary EcologyMicrobial Evolutionta1181AMEBA ACANTHAMOEBA-CASTELLANIILEGIONELLA-PNEUMOPHILABacteriaMEDIA COMPOSITION INFLUENCESPLoS ONE
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α-L-rhamnosidases: Old and New Insights

2007

L-Rhamnose is a component of plant cell wall pectic polysaccharides (Mutter et al., 1994; Ridley et al., 2001), glycoproteins (Haruko and Haruko, 1999) and secondary metabolites such as anthocyanins (Renault et al., 1997), flavonoids (Bar-Peled et al., 1991) and triterpenoids (Friedman and McDonald, 1997). It has also been found in bacterial heteropolysaccharides (Hashimoto and Murata, 1998), rhamnolipids (Ochsner et al., 1994) and in the repeating units of the O-antigen structure of the lipopolysaccharide component of bacterial outer membranes (Chua et al., 1999). Some rhamnosides are important bioactive compounds, e.g. cytotoxic saponins (Bader et al., 1998; Yu et al., 2002), antifungal p…

chemistry.chemical_classificationAntioxidantStereochemistryChemistryRhamnosemedicine.medical_treatmentfood and beveragesGlycosidePolysaccharideCell wallHydrolysischemistry.chemical_compoundTriterpenoidBacterial virulencemedicine
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